本实验对榆树茎皮部分的化学成分进行研究，并考察其抗肿瘤活性。

利用乙醇对榆白皮进行提取，对其乙醇提取物使用二氯甲烷、乙酸乙酯和正丁醇依极性顺序分别进行萃取。利用正反相硅胶、中压液相色谱仪和高压制备型液相色谱仪等技术对榆白皮的乙酸乙酯萃取物进行分离和纯化。利用核磁共振波谱技术对分离得到的化合物进行结构鉴定。为了研究化合物的抗肿瘤活性，利用四甲基偶氮唑蓝MTT法分别对分离得到的化合物进行了人肺癌细胞（A549）生长抑制作用的测定。

从榆白皮中分离得到10个单体化合物，分别为maslinic acid (1), arjunolic acid (2), oleanolic acid (3), naringenin (4), kaempferol (5), isorhamnetin (6), isolariciresinol (7), (+)-lyoniresinol (8), icariside E3 (9), (7Z)- tetradec-7-en-1-ol (10)。其中化合物7、8是首次从榆科中分离得到，化合物5、6、9是首次从该植物中分离得到。化合物1—3对人肺癌细胞（A549）具有显著的抑制作用，化合物5和6对人肺癌细胞（A549）也可在一定程度上对人肺癌细胞（A549）产生抑制作用。

In this experiment, we studied the chemical constituents of the stem bark of Ulmus pumila L. and investigate their anti-tumor activity.

The stem bark of U. pumila. was extracted with ethanol, then the ethanol extract was partitioned sequentially by dichloromethane, ethyl acetate and n-butanol. The ethyl acetate extract extract of the stem bark of U. pumila were separated and purified by the technology of normal silica gel column and reversed-phase silica gel column, medium pressure liquid chromatography and high pressure preparation liquid chromatography, respectively. The chemical structures of the isolated compounds were determined by NMR spectroscopy. In order to study the anti-tumor activity of the isolated compounds, they were evaluated for growth inhibition in human lung cancer cells (A549) using the MTT method.

Ten compounds were isolated from the stem bark of U. pumila. Their structures were determined as maslinic acid (1), arjunolic acid (2), oleanolic acid (3), naringenin (4), kaempferol (5), isorhamnetin (6), isolariciresinol (7), (+)-lyoniresinol (8), icariside E3 (9), (7Z)- tetradec-7-en-1-ol (10). Among them, compounds 7 and 8 were isolated from the family Ulmaceae for the first time, and compounds 5, 6 and 9 were isolated from the stem bark of this plant for the first time. Compounds 1—3 showed significant inhibitory effects on the growth of A549 cells, and compounds 5 and 6 showed weak inhibitory activity against A549 cells.

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